High-Salt Enhances the Inflammatory Response by Retina Pigment Epithelium Cells following Lipopolysaccharide Stimulation

Figure 5

NaCl promoted the expression of NFAT5 and SGK1 on mRNA level in ARPE-19 cells. Cells were stimulated by LPS and incubated with medium alone or additional different concentrations (20 mM or 40 mM) of NaCl for 3 h following preculturing in serum-free medium. Then RNA was isolated and the expression of NFAT5 (a) and SGK1 (b) was measured by RT-PCR. and compared to the control. All results are analyzed following three separate experiments, . Paired-samples t-test (when the difference between the two tested groups conforms to normal distribution) or Wilcoxon matched-pairs test (when the difference between the two tested groups does not conform to normal distribution) was used for statistical analyses for LPS control versus LPS + 20 mM NaCl or LPS + 40 mM NaCl in each group.

(a)

(b)