Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes

Figure 2

Production of TNF-α ((a) and (b)), IL-10 ((c) and (d)), IL-12 (e), and IL-15 (f) in CD14⁺ monocytes treated with IL-18 (100 ng/mL) or IL-18 (100 ng/mL) plus anti-IL-18 (0,5 μg/mL), for 18 hours in culture supernatants, evaluated by ELISA ((a), (c), and (f)), and intracellular staining, by flow cytometry ((b), (d), and (e)). Box-and-whisker plot showing data distribution of 15 healthy subjects tested. Horizontal lines represent the median values; boxes represent the 25th to 75th percentiles and vertical lines the 10th to 90th percentiles. Statistical significance between groups is indicated (  × other groups).

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