LPA Promotes T Cell Recruitment through Synthesis of CXCL13

Figure 4

Effect of the CXCL13 neutralizing antibody on LPA-mediated lymphocyte recruitment into TNF-α-primed air pouches. Air pouch tissues were pretreated with TNF-α (50 ng) for 16 h prior to administration of LPA (3 μg). Where indicated, the anti-CXCL13 neutralizing antibody or the isotype control antibody (IgG2A, 10 μg) was administered into the air pouches 15 min prior to stimulation with LPA for 4 h. The absolute numbers of CD3⁺ T cells in air pouch lavage fluids were determined by flow cytometry as described in Section 2. Data are the mean ± SE of at least 5 mice/group. by analysis of variance.