Claudin-4 Undergoes Age-Dependent Change in Cellular Localization on Pig Jejunal Villous Epithelial Cells, Independent of Bacterial Colonization

Figure 3

Claudin-4 surface localization does not change over time in piglet ileum. Claudin-4 is highly expressed on the surface of ileal intestinal enterocytes with the highest expression on the surfaces between adjacent cells at 24 hours of age ((a), (b)), 48 hours of age ((c), (d)), 3 days of age ((e), (f)), 5 days of age ((g), (h)), and 6 weeks of age ((i), (j)). These images are representatives of IHC performed on tissue from 3 animals per time point. Primary antibody: rabbit anti-Claudin-4. Secondary antibody: FITC-labeled goat anti-rabbit (green). Nuclear stain: DAPI (blue). Scale bar represents 40 μm.

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