Claudin-4 Undergoes Age-Dependent Change in Cellular Localization on Pig Jejunal Villous Epithelial Cells, Independent of Bacterial Colonization

Figure 5

Claudin-4 surface localization in the piglet jejunum is not influenced by colonization by commensal microbiota.In the jejunum of 14-day-old germ-free piglets ((a), (b)), piglets colonized with EC ((c), (d)), LF ((e), (f)), or conventional microbiota (SF; (g), (h)), Claudin-4 is localized to the surface between adjacent enterocytes. These images are representatives of IHC performed on tissue from 4 animals per time point. Primary antibody: rabbit anti-Claudin-4. Secondary antibody: FITC-labeled goat anti-rabbit (green). Nuclear stain: DAPI (blue). Scale bar represents 40 μm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)