Ouabain Modulates Zymosan-Induced Peritonitis in Mice

Figure 3

Effect of ouabain on peritoneal cavity leukocyte subsets. Cells were obtained from peritoneal lavage, 4 and 24 h after the injection of the stimuli i.p. Neutrophils, monocytes, and macrophages were distinguished by flow cytometry using anti-Gr-1 and anti-Mac-3 antibodies. (a) Representative experiment showing the patterns of Gr-1⁺ and Mac-3 expression, as well as the leukocyte populations defined by these molecules. Values of the percentage of neutrophils (Gr-1⁺), macrophages (Mac-3⁺), and monocytes (Gr-1⁺ and Mac-3⁺) were also added to the dot plots. ((b), (c), and (d)) Data were expressed as mean ± SEM and analyzed by Graphpad Prism using ANOVA with Tukey’s posttest, where all groups were compared. The results were obtained from 6 animals per group. and versus PBS group; versus zymosan group.

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