Mediators of Inflammation / 2015 / Article / Fig 3

Research Article

Ouabain Modulates Zymosan-Induced Peritonitis in Mice

Figure 3

Effect of ouabain on peritoneal cavity leukocyte subsets. Cells were obtained from peritoneal lavage, 4 and 24 h after the injection of the stimuli i.p. Neutrophils, monocytes, and macrophages were distinguished by flow cytometry using anti-Gr-1 and anti-Mac-3 antibodies. (a) Representative experiment showing the patterns of Gr-1+ and Mac-3 expression, as well as the leukocyte populations defined by these molecules. Values of the percentage of neutrophils (Gr-1+), macrophages (Mac-3+), and monocytes (Gr-1+ and Mac-3+) were also added to the dot plots. ((b), (c), and (d)) Data were expressed as mean ± SEM and analyzed by Graphpad Prism using ANOVA with Tukey’s posttest, where all groups were compared. The results were obtained from 6 animals per group. and versus PBS group; versus zymosan group.
(a)
(b)
(c)
(d)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.