Mediators of Inflammation / 2015 / Article / Fig 4

Research Article

Ouabain Modulates Zymosan-Induced Peritonitis in Mice

Figure 4

Effect of ouabain on proinflammatory and anti-inflammatory cytokines levels. Swiss mice () were treated for three consecutive days with 0.56 mg/kg of ouabain or vehicle (PBS). After 4 and 24 h stimulation with zymosan, the peritoneal exudate was collected, centrifuged, and stored at −20°C until cytokine assay. Levels of IL-1β (a), TNF-α (b), IL-6 (c), and IL-10 (d) were measured in the exudate supernatant by ELISA. Results were expressed as mean ± SEM and analyzed by Graphpad Prism using ANOVA with Tukey’s posttest, where all groups were compared. versus PBS group; and versus zymosan group.
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