Ouabain Modulates Zymosan-Induced Peritonitis in Mice

Figure 7

Effect of ouabain on in vitro dextran endocytosis by resident peritoneal leukocytes. ((a), (b)) Resident peritoneal cells were incubated with 1, 10, and 100 nM ouabain and 0.5 mg/mL of FITC-conjugated dextran for 1 hour at 37°C or 4°C (control endocytic activity), and fluorescence signals were analyzed by flow cytometry. Scatter plots indicate the percentage of dextran-FITC⁺ cells (a) and the median fluorescence intensity of dextran-FITC (b). ((c), (d)) Prior to the endocytic assay, resident peritoneal cells were cultured in the absence or presence of ouabain for 24 hours. After this culture period, these cells were then incubated for 1 hour with FITC-conjugated dextran at 37°C or 4°C, and fluorescence signals were analyzed by flow cytometry. Scatter plots indicate the percentage of dextran-FITC⁺ cells (c) and the median fluorescence intensity of dextran-FITC (d). Lines refer to the median of the results obtained from at least 7 animals per group.

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