Rapamycin Improves Palmitate-Induced ER Stress/NF κ B Pathways Associated with Stimulating Autophagy in Adipocytes

Figure 5

Rapamycin inhibits PA-induced inflammatory gene expression via depressing NFκB pathways. (a) Mature 3T3-L1 adipocytes were pretreated with or without RAP (100 nM) and with 0.5% BSA or BSA-conjugated PA (0.5 mM) for 12 h. (a) Expression of nuclear and cytoplasmic p65 and p S6 are determined by Western blotting. Lamin/C and β-actin are included as loading controls. (b) Mature adipocytes were pretreatment with NKi (NF-κB inhibitor, BAY11-7082 10 μM) followed by PA treatment for 12 h. mRNA levels of cytokines and chemokines were determined by qPCR. Data are presented as mean ± SEM of three to five separate experiments. versus nontreatment group (0.5% BSA); ^# versus PA 0.5 mM group.

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