Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells

Figure 2

DENV infection promotes NF-κB nuclear translocation and activation. (a) NF-κB reporter assay quantified activation of NF-κB in DENV 2-infected cells for the indicated MOIs 6 h after infection. (b) Immunostaining followed by immunofluorescence microscopy reveals the expression and intracellular localization of the NF-κB p65 (green) and DENV E protein (red) 6 h after infection with DENV 2; DAPI (blue) was used for nuclear staining. Images are representative of three independent experiments. (c) NF-κB reporter assay quantified activation of NF-κB in DENV 2-infected cells pretreated with CAPE. DMSO was used for the negative control. and , compared with untreated cells. , compared with DENV. ns: not significant. The quantitative data shown represent the mean ± SD values of three independent experiments.

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