Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells

Figure 3

NF-κB activation determines TNF-α, NO, and iNOS expression during DENV infection. ELISA (a), Griess’ reagent (b), and western blot analysis (c) quantified expression of TNF-α and iNOS/NO in DENV 2-infected cells pretreated with CAPE. DMSO was used for the negative control. and , compared with untreated cells. , compared with DENV. ns: not significant. For western blot results, one set of representative data obtained from three independent experiments is shown. The relative ratio to β-actin based on densitometer quantification and analysis using ImageJ software is shown. For all experiments, the quantitative data shown represent the mean ± SD values of three independent experiments.

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