Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells

Figure 5

Heat-inactivated DENV does not efficiently cause NF-κB activation, TNF-α production, or iNOS/NO biosynthesis. NF-κB reporter assay (a), ELISA (b), Griess’ reagent (c), and western blot analysis (d) quantified the activation of NF-κB and the expression of TNF-α and iNOS/NO in DENV 2-infected and heat-inactivated DENV 2-treated RAW264.7 cells. , , and , compared with untreated cells. , compared with DENV. For western blot results, one set of representative data obtained from three independent experiments is shown. The relative ratio to β-actin based on densitometer quantification and analysis using ImageJ software is shown. For all experiments, the quantitative data shown represent the mean ± SD values of three independent experiments.

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