Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells

Figure 6

DENV infection induces TLR3-regulated NO and iNOS expression, but not TNF-α expression, following NF-κB activation. (a) In the absence and presence of CAPE, an NF-κB reporter assay was performed to measure NF-κB activation in Poly(I:C)-treated RAW264.7 cells. NF-κB reporter assay (b), ELISA (c), Griess reagent (d), and western blot analysis (e) quantified the activation of NF-κB and the expression of TNF-α and iNOS/NO in DENV 2-infected RAW264.7 cells pretreated with TLR3 inhibitor. , , and , compared with untreated cells. , compared with Poly(I:C) or DENV. ns: not significant. For western blot results, one set of representative data obtained from three independent experiments is shown. The relative ratio to β-actin based on densitometer quantification and analysis using ImageJ software is shown. For all experiments, the quantitative data shown represent the mean ± SD values of three independent experiments.

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