Maresin 1 Inhibits TRPV1 in Temporomandibular Joint-Related Trigeminal Nociceptive Neurons and TMJ Inflammation-Induced Synaptic Plasticity in the Trigeminal Nucleus

Figure 3

Voltage-clamp recordings and single-cell RT-PCR in five DiI-labeled TG neurons. (a) Representative DiI-labeled large-, medium-, and small-sized TG neurons are visualized under phase-contrast (left) and fluorescent (middle) images, respectively. The right image shows the whole-cell patch clamp recording mode in DiI-labeled neurons. Capsaicin (CAP, 100 nM) did not induce inward currents in DiI-labeled large-sized neuron (#1). Three neurons (#2, #3, and #4) responded to capsaicin with inward currents, which were completely blocked by maresin 1 (0.35 nM). Note that maresin 1 (0.35 nM) did not inhibit AITC-induced (300 μM) TRPA1 currents in DiI-labeled small-sized neuron (#5). (b) Single-cell RT-PCR analysis following voltage-clamp recording in five DiI-labeled TG neurons. GAPDH is the positive control. is the negative control.

(a)

(b)