Helicobacter pylori Outer Membrane Vesicle Proteins Induce Human Eosinophil Degranulation via a β2 Integrin CD11/CD18- and ICAM-1-Dependent Mechanism

Figure 2

ECP release from eosinophils stimulated with intact H. pylori OMVs or individual components of OMVs. (a) ECP release is induced in a dose-dependent manner by H. pylori OMVs. Freshly isolated human eosinophils were treated with the indicated concentrations of H. pylori OMVs for 24 h. (b) Human eosinophils were treated with H. pylori LPS (1,000 ng/mL), heat-treated OMVs (200 μg/mL), intact OMVs (200 μg/mL), or purified VacA (1,000 ng/mL) for 24 h. The concentration of ECP in each culture supernatant was determined by ELISA (mean ± SEM, ). ; NS, statistically nonsignificant.

(a)

(b)