Helicobacter pylori Outer Membrane Vesicle Proteins Induce Human Eosinophil Degranulation via a β2 Integrin CD11/CD18- and ICAM-1-Dependent Mechanism

Figure 3

Effects of H. pylori OMV-exposed gastric epithelial cells on ECP released from eosinophils. (a) Primary human gastric epithelial cells (GECs) were exposed to either OMVs (200 μg/mL) or heat-treated OMVs (200 μg/mL) for 24 h and then washed twice in PBS. Human eosinophils were cocultured with either OMV-exposed or -unexposed GECs for 24 h. The concentrations of ECP in the culture supernatants were measured by ELISA (mean ± SEM, ). ; NS, statistically nonsignificant. (b) Primary human GECs were cultured for 24 h in either the presence or absence of OMVs (200 μg/mL). Culture supernatants were then collected as described in Materials and Methods. GECs were stimulated with control-CM (50% v/v) or OMV-CM (50% v/v) for 24 h and then washed twice in PBS. Finally, control-CM-exposed or OMV-CM-exposed GECs were cocultured with human eosinophils for 24 h. The concentrations of ECP in the culture supernatants were measured by ELISA (mean ± SEM, ). .

(a)

(b)