Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy

Figure 3

Immunostaining and quantification of cardiac MSC related to cardiac inflammation and expression of SDF-1α mRNA. (a) Representative image of immunofluorescence of MSC in EMB. Serial sections were stained with (A) an overlay of α-sarcomeric actin (red), nuclear counterstained (DAPI, blue), anti-CD105 and anti-CD90 (red, and B), and anti-CD45 (green, and C) as a lymphocytes marker (the arrow image (A) highlights the MSC). (b) Quantitative analysis of CD45⁻CD34⁻CD105⁺CD90⁺-MSC in EMB of patients without () and with () cardiac inflammation, as defined by the number of LFA-positive cells (box plots are given as median and IQR; whiskers represent 95% CI). (c) Quantitative analysis of CD45⁻CD34⁻CD105⁺CD90⁺-MSC in EMB after dichotomisation with respect to SDF-1α mRNA expression in their EMB (cut-off: median, box plots are given for median and IQR; whiskers represent 95% CI). (d) Correlation between the transcardiac gradient of circulating MSC and MSC in EMB. (e) Representative image of immunofluorescence of MSC and proliferation in EMB. The same serial sections as indicated above (3(a)) were stained with (A) anti-Ki67 (yellow) for proliferation, (B) overlay of CD105 (red), anti-CD45 (green), and Ki67 (yellow), and (C) overlay of α-sarcomeric actin (red), nuclei (DAPI, blue), CD105 (red), anti-CD45 (green), and Ki67 (yellow).

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