Anti-Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study

Figure 3

IL-8 gene expression, analyzed through qRT-PCR, in HaCaT cells stimulated with LPS (25 μg/mL) and incubated in pretreatment, concomitant, and posttreatment settings with low (0.3 mg/μL; 0.6 mg/μL) and high (1.5 mg/μL; 3 mg/μL) doxycycline doses. IL-8 gene expression in (a) cells stimulated with LPS for 24 h and successively incubated with doxycycline for further 24 h, mRNA extraction performed at 48 h; (b) cells incubated with doxycycline immediately after LPS, mRNA extraction performed at 24 h; (c) cells pretreated with doxycycline for 24 h and successively stimulated with LPS; mRNA extraction at 48 h (, , and ; ns: not statistically significant).

(a)

(b)

(c)