Cannabinoid Receptor CB2 Is Involved in Tetrahydrocannabinol-Induced Anti-Inflammation against Lipopolysaccharide in MG-63 Cells

Figure 3

THC upregulated CB2 receptor expression. Immunofluorescent staining (a) and western blot (b) were used to determine the CB2 protein expression. The cells were treated with or without 10 ng/mL LPS for 24 h in the presence or absence of 5 μM THC or 10 μM AM630 (CB2 receptor antagonist). The CB2 receptor expression (red) was observed by a confocal microscope, and stronger signal indicated higher expression of CB2 protein. Nuclei were counter-stained with DAPI (blue). Bar = 10 μm. Results are means ± SD (). : ; NS: no significance.

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