A Subgroup of Latently Mycobacterium tuberculosis Infected Individuals Is Characterized by Consistently Elevated IgA Responses to Several Mycobacterial Antigens

Table 1

Recombinant antigens of M. tuberculosis used in this study.

Protein name(s)	Rv number	Mol mass (kDa)	Expression vector	6-fold His-Tag	E. coli host strain
NarL	Rv0844c	23.9	pET22	N-terminal	BL21 (DE3)
AlaDH	Rv2780	38.7	pJLA604	—	CAG629
19 kDa glycolipoprotein, LpqH	Rv3763	16.0	pET26	C-terminal	BL21 (DE3)
PstS3	Rv0928	38.8	pET22	C-terminal	Rosetta (DE3)
MPT83, MPB83	Rv2873	24.9	pET21	C-terminal	BL21 (DE3) [pLysS]

Nitrate/nitrite response transcriptional regulatory protein NarL; secreted L-alanine dehydrogenase (AlaDH); 19 kDa lipoprotein antigen precursor LpqH; periplasmic phosphate-binding lipoprotein PstS3; cell surface lipoprotein MPT83.