Downregulation of mPGES-1 Expression via EGR1 Plays an Important Role in Inhibition of Caffeine on PGE₂ Synthesis of HBx(+) Hepatocytes

Figure 3

Effect of caffeine on the transcriptional activity of COX-2 and mPGES-1 gene promoter in HBx positive hepatocytes. (a) The COX-2 promoter activity (RLU value) in HL7702 and HL7702-HBx cells. (b) The mPGES-1 promoter activity (RLU value) in HL7702 and HL7702-HBx cells. (c) The COX-2 promoter activity (RLU value) in HepG2 and HepG2-HBx cells. (d) The mPGES-1 promoter activity (RLU value) in HepG2 and HepG2-HBx cells. Cells were cotransfected with 1 μg of pGL3B-COX-2 (COX-2 promoter luciferase reporter plasmid) or pGL3B-628 (mPGES-1 promoter luciferase reporter plasmid) and 100 ng pRL-TK, and pGL3-basic served as the negative control. or versus HL7702 or HepG2 cells (RLU was set to 1, ).

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