Glycyrrhizic Acid Promotes M1 Macrophage Polarization in Murine Bone Marrow-Derived Macrophages Associated with the Activation of JNK and NF-κB

Figure 5

Effect of GA on NO synthesis in BMDMs. BMDMs were treated with GA (25~100 μg/mL). (a) At the indicated time points, total RNA was extracted and the expressions of iNOS and Arg1 were measured by real-time PCR. (b, c) After 24 h of treatment, cells and supernatants were collected, cell lysates were prepared and subjected to western blotting with iNOS and β-actin antibody, and the nitrite generation in the supernatants was quantified as described in Section 2. Data are mean ± SD for three independent experiments. , (-test).

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