Figure 2: Inhibition of Nrf2 suppresses PUN induced HO-1 protein expression in RAW264.7 cells. Cells were treated for 8 h with 100 μM PUN in the presence or absence of 20 nM brusatol at 37°C in a humidified incubator with 5% CO2. The HO-1 and Nrf2 protein expression were analyzed by Western blotting. Data represent the mean ± SEM of three independent experiments and differences between mean values were assessed by one-way ANOVA. and indicate significant differences compared with the control group.