(a)
(b)
Figure 4: PI3K/Akt pathway regulates property of PUN on ROS scavenging. (a) ROS detection was performed using a fluorescence macroscopy. (A) RAW264.7 cells were cultured in DMEM for 12 h and then incubated with probe DCFH2DA for 15 min. (B) RAW264.7 cells were treated with 1 μg/mL LPS for 12 h and then incubated with probe DCFH2DA for 15 min. (C) RAW264.7 cells were pretreated with 100 μM PUN for 1 h and treated with 1 μg/mL LPS for 12 h and then incubated with probe DCFH2DA for 15 min. (D) RAW264.7 cells were pretreated with 100 μM PUN and 20 μM LY294002 for 1 h and treated with 1 μg/mL LPS for 12 h and then incubated with probe DCFH2DA for 15 min. (b) ROS production was measured by a fluorescence microplate reader. RAW264.7 cells were pretreated for 1 h with 100 μM PUN in the presence or absence of 20 μM LY294002 before treatment with 1 μg/mL LPS for 12 h and then incubated with probe DCFH2DA for 15 min. Data represent the mean ± SEM of three independent experiments and differences between mean values were assessed by one-way ANOVA. and indicate significant differences compared with the control group.