Proinflammatory Cytokines Increase Vascular Endothelial Growth Factor Expression in Alveolar Epithelial Cells
Northern blots showing VEGF RNA expression in A549 cells. Graphs below blots indicate fold-change in RNA expression compared to vehicle (Cntrl) and are normalized to β-actin expression. Phorbol ester (PMA) is included as a positive control in (a). Only a single VEGF band was seen at 3.7 kb, corresponding to the mRNA for VEGF165. (a) Results from 6 hours after stimulation with individual proinflammatory cytokines, showing VEGF bands with corresponding β-actin bands; graphic analysis of comparison to vehicle control (cntrl) is at the bottom of figure. (b) Results from 6 hours after stimulation with a mixture of IL-1, IL-6, and IL-8 compared to results with IL-1 alone, showing VEGF bands with corresponding β-actin bands. All conditions demonstrated increased VEGF expression compared to vehicle control (Cntrl), but effects of cytokine mixes are not significantly different than IL-1 alone, though trends are apparent. Graphic analysis is at the bottom of figure. Blots are representative of 6 individual experiments. , , and .