Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model

Figure 4

Production of ROS by M1 and M2 macrophages in response to peritoneal fluid (PF) from septic swine in vitro. The production of ROS by human macrophages was induced in vitro with PF taken from swine at various intervals following the induction of septic injury, and the results are expressed as the difference (Δ) from the initiation of treatment at the 12 hrs interval (), representing a 36 hrs period of treatment. (a) PF from NPT-treated septic swine induced a significant inflammatory response (mean change in nMol/hour/mg protein ± SEM from baseline) in M1 macrophages than PF from SOC-treated septic swine, peaking at 12 hrs after the initiation of treatment (). (b) PF from NPT-treated septic swine induced a greater inflammatory response (mean change in nMol/hour/mg protein ± SEM from baseline) in M2 macrophages than PF from SOC-treated septic swine, peaking at 12 hrs after the initiation of treatment ().

(a)

(b)