Research Article

Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury

Figure 4

Tgm2 protein expression. Changes in the expression levels of Tgm2 were investigated by immunoblotting of lysates of mononuclear intravascular leukocytes isolated from control animals isogenic (Iso) and allogenic (Allo) renal transplants (a) as well as total control and graft tissue lysates (c) on day 42 after transplantation. Detection of the house-keeping enzyme GAPDH was performed for normalization. Densitometric quantifications of the bands are depicted for perfusates (b) and kidney tissues (d) ( per group). All bands detected by antibodies to Tgm2 including the additional low molecular weight bands seen in tissue samples on day 42 (c) were quantified. Box plots indicate median and percentiles 0, 25, 75, and 100. . OD: optical density.
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