Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury

Figure 5

Immunohistochemical detection of Tgm2 and CD163. Immunohistochemical detection of Tgm2 was performed on paraffin sections of control kidneys (a) and isogenic (b) and allogenic (c) renal transplants on day 9 after transplantation. CD163-positive cells were stained on sections of isogenic (e) and allogenic (f) kidney grafts. Tgm2 or CD163 are labeled in brown; sections are lightly stained with hemalum. In (d), double-staining experiments using ED1 antibodies directed to a CD68-like antigen (in blue) and Tgm2 (in brown) reveal that a majority of Tgm2-positive intravascular leukocytes are monocytes. Representative sections out of four individual experiments are shown. The arrow points to a double-positive blood leukocyte.

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