Thiopurines Induce Oxidative Stress in T-Lymphocytes: A Proteomic Approach

Table 1

Differentially regulated proteins identified by mass spectrometry.

Spot number	Protein name^a	Function (COGnitor NCBI and SwissProt)	Expression change (in fold)
			Jurkat wt		Jurkat kd		kd DMSO/wt DMSO	kd 6-MP/wt 6-MP	kd 6-TG/wt 6-TG
			6-MP	6-TG	6-MP	6-TG
	Oxidative stress
8	Thioredoxin (THIO)	Cell redox homeostasis, protein thiol-disulfide exchange	NS	1.67^*↑	NS	NS	NS	NS	NS
12	Thioredoxin domain containing protein 17 (TXD17)	Disulfide reductase, modulates TNF-alpha signaling and NF-kappa-B activation	NS	2.87^*	NS	NS	NS	NS	NS
	Cell cycle regulation
6	Stathmin 1 (STMN1)	Cell differentiation, microtubule depolymerization, intracellular signal transduction	1.53^*	NS	1.53^*	NS	NS	NS	NS
7	Costars family protein ABRACL (ABRAL)	Member of costars family	1.96^*	NS	NS	NS	NS	NS	NS
	Cytoskeleton regulation
5	Cofilin 1 (COF1)	Rho protein signal transduction, actin cytoskeleton organization, antiapoptosis	NS	2.53^*	NS	NS	2.78^**	1.5^*	2.45^*
9	Profilin 1 (PROF1)	Actin cytoskeleton organization, regulation of transcription from mRNA polymerase II promoter	NS	5.25^*	NS	NS	NS	NS	NS
13	Myotrophin (MTPN)	Positive regulation of cardiac muscle hypertrophy, NF-kappa B transcription factor activity	NS	2.02^*	NS	NS	NS	NS	1.75^*
	Enzymes
3	Delta (3,5)-delta(2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1)	Involved in fatty acid metabolism and lipid metabolism	NS	NS	NS	2.22^***	NS	NS	1.54^*
4	Deoxyuridine 5′-triphosphate nucleotidohydrolase, mitochondrial (DUT)	Nucleotide metabolism, dUTP diphosphatase activity	NS	NS	NS	NS	2.02^**	NS	1.78^*
	Others
1	Ataxin 10 (ATX10)	Role in the maintenance of a critical intracellular glycosylation level and homeostasis	NS	NS	NS	NS	2.63^*	NS	NS
2	Heterogenous ribonucleoprotein H3 (HNRH3)	Nuclear mRNA splicing via spliceosome	NS	NS	NS	NS	1.74^*	NS	NS
10	Macrophage migration inhibitory factor (MIF)	Cell proliferation, inflammatory response	NS	3.32^*	NS	1.81^*	NS	NS	NS
11	Hemoglobin subunit alpha (HBA)	Oxygen transport, hydrogen peroxide catabolic process	4.09^*	5.55^*	NS	NS	NS	NS	NS

^aProteins identified by Q-TOF MS/MS analysis and database search against SwissProt; : upregulated; : downregulated; NS: nonsignificant change.
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