Thiopurines Induce Oxidative Stress in T-Lymphocytes: A Proteomic Approach

Table 2

Differentially regulated phosphoproteins identified by mass spectrometry.

Spot number	Protein name^a	Function (COGnitor NCBI and SwissProt)	Phosphorylation change (in fold)
			Jurkat wt		Jurkat kd		kd DMSO/wt DMSO	kd 6-MP/wt 6-MP	kd 6-TG/wt 6-TG
			6-MP	6-TG	6-MP	6-TG
	Oxidative stress
8	Glutathione S-transferase Mu 3 (GSTM3)	Glutathione transferase activity	NS	3.81^**	NS	1.84^*	NS	NS	2.26^*
9	Thioredoxin-dependent peroxide reductase (PRDX3)	Involved in redox regulation of the cell	NS	NS	NS	NS	2.01^*	2.14^***	2.85^***
	Cell cycle regulation
2	T-complex protein 1 subunit zeta (TCPZ)	De novo posttranslational protein folding	NS	NS	1.86^**	NS	NS	1.63^*	NS
4	Rab GDP dissociation inhibitor beta (GDIB)	Regulates the GDP/GTP exchange reaction of most Rab proteins	NS	NS	NS	NS	1.53^*	1.59^**	NS
6	26S protease regulatory subunit 10B (PRS10)	ATP-dependent degradation of ubiquitinated proteins	1.56^**	NS	NS	NS	NS	NS	NS
7	mRNA export factor (RAE1L)	Involved in nucleocytoplasmic transportof mRNA	NS	1.69^**	NS	1.8^*	NS	NS	1.85^*
	Cytoskeleton regulation
3	Coronin-1A (COR1A)	T-cell homeostasis, involved in cell locomotion	1.54^*	NS	NS	NS	NS	NS	NS
5	Actin-related protein 2 (ARP2)	ATP-binding component of the Arp2/3 complex	NS	1.52^*	NS	NS	NS	NS	NS
	Enzymes
1	6-Phosphofructokinase type C (K6PP)	Involved in glycolysis, protein homotetramerization	NS	NS	NS	1.5^*	1.65^*	NS	NS

^aProteins identified by Q-TOF MS/MS analysis and database search against SwissProt; : upregulated; : downregulated; NS: nonsignificant change. , , and .