Chemical Hypoxia Brings to Light Altered Autocrine Sphingosine-1-Phosphate Signalling in Rheumatoid Arthritis Synovial Fibroblasts

Table 1

S1P content in normal FLS and RAFLS.

	S1P content (pmol/mg of protein)
Normal FLS
Donor #1 (S3618)	64.5 ± 1.5
Donor #2 (S3739)	273.0 ± 21
RAFLS
Donor #1 (37158A1-S)	19.5 ± 1.5
Donor #2 (87546A1-S)	22.5 ± 1.5

Cell lysates from human primary FLS of normal () and RA () donors were prepared. S1P content in cell lysates (50 μg protein) was measured using the S1P assay kit from Echelon Inc. according to the manufacturer’s instruction.