AP-1-Targeted Anti-Inflammatory Activities of the Nanostructured, Self-Assembling S5 Peptide

Figure 3

Effects of the S5 peptide on the activation of transcription factors. (a, b, and c) HEK293 cells that had been cotransfected with constructs driving the expression of AP-1-Luc, TLR adaptor molecules (MyD88 and TRIF), and β-gal (as a control) were treated with the S5 peptide (0 to 100 μg/mL) in the presence or absence of PMA (100 nM). The resultant luciferase activity was measured with a luminometer. (d) The nuclear translocation level of a transcription factor in the AP-1 family (c-Jun) was assessed by immunoblotting analysis with antibodies against total c-Jun. and compared with control cells.

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