AP-1-Targeted Anti-Inflammatory Activities of the Nanostructured, Self-Assembling S5 Peptide

Figure 4

Effects of the S5 peptide on the activation of the AP-1 signaling pathway. (a and b) Phosphoprotein and total protein levels of p38, JNK, ERK, MEK1/2, MKK3/6, MKK4/7, TAK1, and β-actin in the lysates of RAW264.7 cells were analyzed by Western blotting. (c) The levels of total and phosphorylated c-Jun, p38, MKK3/6, and β-actin in lysates from peritoneal macrophages were determined by immunoblotting analysis. (d) The effect of the S5 peptide on the c-Jun/p38 interaction was determined by immunoprecipitation analysis with antibody against p38 and by immunoblotting analysis with antibodies against p-c-Jun, c-Jun, and p38.

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