Serum Galectin-9 Levels Are Associated with Coronary Artery Disease in Chinese Individuals

Figure 4

Recombinant Gal-9 enhances the percentage of Tregs and decreases Th17 effector response. CD3/CD28-activated PBMCs were incubated with either medium or increasing concentrations of recombinant Gal-9 for 24 h. The numbers of activated Th1 cells (CD4⁺IFN-γ⁺; (a)), Th2 cells (CD4⁺IL-4⁺; (b)), Th17 cells (CD4⁺IL-17⁺; (c)), and Tregs (CD4⁺CD25⁺Foxp3⁺; (d)) were determined by FACS. Gal-9 increased the development of Tregs and decreases the percentage of Th17 cells dose dependently. (e) Cytokines produced by Gal-9 exposed PBMC were measured in the supernatant by ELISA. Data represent independent PBMCs of NCA. , , and compared with control.

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