Deficits in Endogenous Adenosine Formation by Ecto-5′-Nucleotidase/CD73 Impair Neuromuscular Transmission and Immune Competence in Experimental Autoimmune Myasthenia Gravis

Figure 5

The amount ADO released upon phrenic nerve stimulation is lower in EAMG animals. The ordinates represent the (a) evoked release of ADO upon phrenic nerve trunk electrical stimulation (750 pulses applied at 5 Hz frequency) and (b) basal ADO quantified by HPLC(diode array detection). Nerve-evoked release of ADO was calculated by subtracting the basal release, measured in the sample collected before stimulation, from the total release of adenosine determined after stimulus application. The data are means S.E.M. of 13 animals of each group (naïve, control, and EAMG).

(a)

(b)