Mediators of Inflammation

Review Article

The Contribution of the Airway Epithelial Cell to Host Defense

Table 1

Effect of cytokines on ion transport in airway epithelial cells.


Model system	Cytokine	Ion channel	Techniques	Principle findings	Reference	EPub

Rabbit	TGF	ENaC	²²Na⁺ efflux, electrophysiology, flow cytometry, surface biotinylation, and RT-PCR	TGF decreases ENaC-mediated sodium and fluid uptake	[25]	2014

Air liquid epithelial cell culture with human CFBE41o⁻ and transfected cells	IL6, IL8, and CXCL1/2	CFTR, TMEM16A	Electrophysiology, immunocytochemistry, human cytokine antibody array, and cell-surface ELISA	CFTR and TMEM16A decrease secretion of IL6, IL8, and CXCL1/2	[24]	2012

Slc26a9-deficient mice	IL13	Slc26a9	Electrophysiology	IL13 increases Slc26a9-mediated Cl⁻ secretion	[23]	2012

BALB/c mice	IL13	ENaC	Electrophysiology, RT-PCR	IL13 decreases ENaC expression	[22]	2010

Human bronchial epithelial cells	IL17A	Bicarbonate transport	Electrophysiology, measurement of surface and intracellular pH	IL17A increases secretion	[21]	2009

Human nasal epithelial cells	IL13	CFTR	Immunocytochemistry, western blot	IL13 increases CFTR expression	[20]	2007

Primary culture of rat alveolar epithelial type II cells, primary culture of human alveolar epithelial type II cells	Il1	ENaC	²²Na⁺ efflux, electrophysiology, western blot, and RT-PCR	IL1 decreases ENaC expression	[18]	2005

Rat alveolar epithelial cells	TNF	ENaC	Electrophysiology, northern blot, and western blot	TNF decreases ENaC expression	[19]	2004

Primary culture of rat alveolar epithelial type II cells, primary culture of human alveolar epithelial type II cells	TGF	ENaC	²²Na⁺ efflux, electrophysiology, western blot, and RT-PCR	TGF decreases ENaC expression	[15]	2003

Primary culture of human bronchial epithelial cells	IL4, IL13	CFTR, ENaC	Electrophysiology, western blot	IL4 and IL13 increase CFTR expression IL4 and IL13 decrease ENaC expression	[16]	2002

Primary culture of human bronchial epithelial cells	IFN, TNF	ENaC, CFTR	Electrophysiology, western blot, and transepithelial fluid transport	IFN decreases CFTR expression IFN decreases ENaC-mediated sodium transport TNF increases CFTR expression	[17]	2000