Cardiac-Restricted IGF-1Ea Overexpression Reduces the Early Accumulation of Inflammatory Myeloid Cells and Mediates Expression of Extracellular Matrix Remodelling Genes after Myocardial Infarction
Positive remodeling in the αMHC.IGF-1Ea infarcted region after myocardial infarction. Quantification of the infarcted area at (a) 3 days and (b) 28 days after MI. . Representative histological sections of (c) WT and (d) αMHC.IGF-1Ea hearts stained with Masson’s trichrome 28 days after MI. Scale bar: 1 mm. ((e)–(l)) mRNA relative expression of (e) transforming growth factor beta (TGF-β), (f) matrix metalloproteinase 9 (MMP-9), (g) matrix metalloproteinase 2 (MMP-2), (h) tissue inhibitor of metalloproteinase (TIMP) 2, (i) TIMP-1, (j) collagen (Col) Iα1, (k) Col Iα3, (l) ColIα1/ColIα3 ratio, (m) lysyl oxidase in the infarct area. Results are expressed as mean fold induction ± SEM. Solid lines represent WT mice. Dashed lines represent αMHC.IGF-1Ea mice. per group. Two-tailed Student’s t-test was performed to compare WT versus αMHC.IGF-1Ea at selected time points after MI. , , .