Mediators of Inflammation / 2015 / Article / Fig 4

Research Article

Cardiac-Restricted IGF-1Ea Overexpression Reduces the Early Accumulation of Inflammatory Myeloid Cells and Mediates Expression of Extracellular Matrix Remodelling Genes after Myocardial Infarction

Figure 4

Characterisation and temporal dynamics of immune cell populations in αMHC.IGF-1Ea hearts after MI. Quantification of (a) total leukocytes (CD45+), (b) neutrophils (CD45+, CD11b+, F4/80−, CD11c−, and Ly6G+), and (c) monocytes (CD45+, CD11b+, CD11c−, Ly6G−, and F4/80−) which were further classified as (d) and (e) monocytes. (f) Macrophages (CD45+, CD11b+, CD11c−, Ly6G−, and F4/80+) were further characterised on the basis of Ly6C and CD206 expression as (g) / (inflammatory macrophages) and (h) / (reparative macrophages). For this work, only cells that were either /CD206− or Ly6C−/CD206+ were analysed, although we noted a double positive cell population (i.e., CD206+, Ly6C+). (i) Dendritic cells were defined as CD45+, CD11b+, F4/80−, CD11c+, and Ly6G−. Data is presented as the total number of cells per mg of heart. –6 per group. Two-tailed Student’s t-test was performed to compare WT versus αMHC.IGF-1Ea at selected time points after MI. , .
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