Cardiac-Restricted IGF-1Ea Overexpression Reduces the Early Accumulation of Inflammatory Myeloid Cells and Mediates Expression of Extracellular Matrix Remodelling Genes after Myocardial Infarction
Characterisation and temporal dynamics of immune cell populations in αMHC.IGF-1Ea hearts after MI. Quantification of (a) total leukocytes (CD45+), (b) neutrophils (CD45+, CD11b+, F4/80−, CD11c−, and Ly6G+), and (c) monocytes (CD45+, CD11b+, CD11c−, Ly6G−, and F4/80−) which were further classified as (d) and (e) monocytes. (f) Macrophages (CD45+, CD11b+, CD11c−, Ly6G−, and F4/80+) were further characterised on the basis of Ly6C and CD206 expression as (g) / (inflammatory macrophages) and (h) / (reparative macrophages). For this work, only cells that were either /CD206− or Ly6C−/CD206+ were analysed, although we noted a double positive cell population (i.e., CD206+, Ly6C+). (i) Dendritic cells were defined as CD45+, CD11b+, F4/80−, CD11c+, and Ly6G−. Data is presented as the total number of cells per mg of heart. –6 per group. Two-tailed Student’s t-test was performed to compare WT versus αMHC.IGF-1Ea at selected time points after MI. , .
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