Activation of CXCL-8 Transcription by Hepatitis E Virus ORF-1 via AP-1

Figure 2

CXCL-8 transcriptional activation by HEV ORF-1. (a) HuH-7 cells were cotransfected with plasmid vector, or plasmids expressing HEV ORF-1, ORF-2, or ORF-3 proteins and the −173 CXCL-8 promoter-luciferase reporter. Luciferase assay was performed 48 hours after transfection. Luciferase activity was normalized against the protein concentration of the same samples. : p ≤ 0.05, : p ≤ 0.01, and NS: not significant by Student’s t-test. (b) The expression of HEV ORF-1, ORF-2, or ORF-3 proteins with a N-terminal Flag-tag after transfection into HEK293 cells was analyzed by immunoprecipitation followed by Western blotting with an anti-Flag antibody. The location of HEV proteins was indicated by in the blot.

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