Activation of CXCL-8 Transcription by Hepatitis E Virus ORF-1 via AP-1

Figure 3

AP-1 is involved in CXCL-8 activation by HEV ORF-1. HuH-7 cells were cotransfected with plasmid vector or a plasmid expressing HEV ORF-1 protein, together with −173 mAP-1 or mNRE CXCL-8 promoter-luciferase reporters (a) or a luciferase reporter with seven repeats of the AP-1 binding sequences (b). Luciferase assay was performed 48 hours after transfection. Luciferase activity was normalized against the protein concentration of the same samples. : p ≤ 0.01; NS: not significant by Student’s t-test.

(a)

(b)