Activation of CXCL-8 Transcription by Hepatitis E Virus ORF-1 via AP-1

Figure 4

AP-1 is required for CXCL-8 activation by HEV genomic RNA. HuH-7 cells were cotransfected with HEV genomic RNA, together with the wild-type or mAP-1 −173 CXCL-8 promoter-luciferase reporters. Luciferase assay was performed 48 hours after transfection. Luciferase activity was normalized against the protein concentration of the same samples. : p ≤ 0.01 by Student’s t-test.