CD-40 (green fluorescence) colocalization with markers of neuronal cells in the injured cortex. ((a)–(c)) Double-immunofluorescence analysis of CD40 (a) and MAP-2 ((b) red fluorescence) revealed that CD40 fluorescence signal was mostly detected on neuronal cell bodies ((c) yellow fluorescence). ((d)–(f)) CD40 (d) and NeuN ((e) red fluorescence) fluorescence colocalized not only on neuronal cell bodies but also on axons (f). Arrow head points to varicosities along axons. Scale bar = 5 μm.