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Mediators of Inflammation
Volume 2015, Article ID 498405, 17 pages
http://dx.doi.org/10.1155/2015/498405
Research Article

Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and Suppresses Expression of Inflammatory Mediators in the Rat Model of Brain Injury

1Department of Neurobiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, 11060 Belgrade, Serbia
2Institute of Medical Physiology “Richard Burian”, School of Medicine, University of Belgrade, 11000 Belgrade, Serbia
3Centre for Hyperbaric Medicine, 11040 Belgrade, Serbia
4Institute of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, 11001 Belgrade, Serbia

Received 3 December 2014; Revised 5 February 2015; Accepted 8 March 2015

Academic Editor: Amos Douvdevani

Copyright © 2015 Irena Lavrnja et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Staining pattern of GFAP, vimentin (VIM) and ICAM-1 in the left cortex of control group (C, intact rats), control HBO group (CHBO, intact rats subjected to the HBO protocol for 10 consecutive days), sham group (S, the animals that underwent surgical procedure without skull opening and sacrificed 10 days post operation) and sham HBO group (SHBO, the animals that underwent sham surgery and were subjected to the HBO protocol for 10 consecutive days). Sham operation and/or the treatment with HBO did not significantly change the GFAP, VIM and ICAM-1 staining pattern. Scale bar = 50 µm.

Supplementary Figure 2: Staining pattern of CD40 and CD40L in the left cortex of control group (C, intact rats), control HBO group (CHBO, intact rats subjected to the HBO protocol for 10 consecutive days), sham group (S, the animals that underwent surgical procedure without skull opening and sacrificed 10 days post operation) and sham HBO group (SHBO, the animals that underwent sham surgery and were subjected to the HBO protocol for 10 consecutive days). Staining pattern of CD40 i CD40L was not significantly changed after the sham operation and/or treatment with HBO. Scale bar = 50 µm.

Supplementary Figure 3: Double immunofluorescence analysis of CD40L (green) and GFAP (red) colocalization in the left cortex of control group (C, intact rats), control HBO group (CHBO, intact rats subjected to the HBO protocol for 10 consecutive days), sham group (S, the animals that underwent surgical procedure without skull opening and sacrificed 10 days post operation) and sham HBO group (SHBO, the animals that underwent sham surgery and were subjected to the HBO protocol for 10 consecutive days). Paucity of CD40L/GFAP (yellow fluorescence) positive fibrous astrocytes was seen throughout the left cortex. Morphology of these astrocytes was not significantly changed after the sham operation and/or treatment with HBO. Scale bar = 50 µm.

  1. Supplementary Material