TGF-Beta Blockade Increases Renal Inflammation Caused by the C-Terminal Module of the CCN2

Figure 1

TGF-β blockade exacerbates CCN2(IV)-induced renal damage. C57BL/6 mice received a single ip injection of 2.5 ng/g body weight recombinant CCN2(IV) or vehicle (saline) and were sacrificed after 10 days. For TGF-β neutralization experiments, mice were treated with anti-TGF-β antibody or an isotype IgG control ( mice per group) starting 24 hours before CCN2(IV) injection and every 72 h until sacrifice at day 10 after CCN2(IV). Renal damage was assessed by evaluation of renal levels of biomarker NGAL by western blot. (a) shows a representative western blot and data expressed as mean ± SEM ( animals per group) of fold-change as compared to controls. versus control.

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