Vitamin D Potentiates the Inhibitory Effect of MicroRNA-130a in Hepatitis C Virus Replication Independent of Type I Interferon Signaling Pathway

Figure 4

Calcitriol does not increase the miR-130a-induced IFN and IFN upregulation. Huh7.5.1 cells infected with J6/JFH1 infected HCVcc were transfected with 2 nM miR-130a mimic or treated with 0.1 μM calcitriol or both and the expressions of IFN (a) and IFN (b) mRNAs were quantified by quantitative RT-PCR 48 h after transfection as described in Section 2. Data are presented as , . versus control and mock and Nc (microRNA mimic negative control).

(a)

(b)