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Mediators of Inflammation
Volume 2015, Article ID 508989, 5 pages
Research Article

Vitamin D Potentiates the Inhibitory Effect of MicroRNA-130a in Hepatitis C Virus Replication Independent of Type I Interferon Signaling Pathway

1Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China
2Guangzhou No. 8 People’s Hospital, Guangzhou, China
3Toronto General Research Institute, University of Toronto, Toronto, ON, Canada

Received 15 December 2014; Revised 7 April 2015; Accepted 15 April 2015

Academic Editor: Stuart Gray

Copyright © 2015 Xiaoqiong Duan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Ethanol has no effect on the expressions of HCV RNA and IFNα/β at the concentration of 0.02%.

As the calcitriol was dissolved in 100% ethanol at the stock concentration of 500 ωM and used at the concentration of 0.1ωM, the final concentration of ethanol in our experiment was 0.02%. In order to make sure there is no effect of 0.02% ethanol on HCV replication and IFNα/β expression, Con1b replicon cells and Huh7.5.1 cells infected with J6/JFH1 HCVcc were seeded in a 24-well plate for overnight and 5 ul of 2% ethanol was added into each well (final concentration 0.02%). Total RNAs were extracted 48h post treatment and expression levels of HCV RNA, IFNα and IFNβ mRNAs were quantified by RT-PCR. The results showed that ethanol has no effect on the expressions of HCV RNA and IFNα/β at the concentration of 0.02%.

  1. Supplementary Material