Protective Effects of BDNF against C-Reactive Protein-Induced Inflammation in Women

Figure 3

BDNF reduces CRP levels in cells. (a) HUVECs, HMVEC-C, or HepG2 cells were lysed, separated by SDS-PAGE and probed with anti-TrkB, anti-CRP, and anti-actin antibodies as a loading control. (b) HUVECs were untreated or treated with the indicated concentrations of BDNF for 18 hrs and CRP mRNA levels were assessed by RT-qPCR and normalized to GAPDH. (c) Cells treated as in (b) were lysed and probed with anti-CRP and anti-actin antibodies as a protein loading control. (d) CRP protein levels were quantified from immunoblots and normalized to actin. The histograms represent the normalized mean + SEM from 3 independent experiments. , , and using one-way ANOVA and Tukey’s post hoc test.

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