Protective Effects of BDNF against C-Reactive Protein-Induced Inflammation in Women

Figure 4

CRP or BDNF treatment does not affect BDNF or APE1 levels, respectively. (a) HUVECs or HepG2 cells were untreated or treated with the indicated concentrations of BDNF for 18 hrs. Cells were lysed, separated by SDS-PAGE, and probed with anti-APE1 and anti-actin antibodies. (b) APE1 protein levels were quantified from immunoblots and normalized to actin. The histograms represent the normalized mean + SEM ( for HepG2 and for HUVEC). (c) HUVECs were treated for 18 hrs with 25 μg/mL CRP. Cells were lysed, separated by SDS-PAGE, and probed with anti-BDNF and anti-actin antibodies as a loading control. Mature BDNF (mBDNF) is indicated. (d) BDNF protein levels were quantified from immunoblots and normalized to actin ().

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