Cannabidiol Rescues Acute Hepatic Toxicity and Seizure Induced by Cocaine

Figure 7

Cannabidiol (CBD; 30 mg/Kg) prevented cocaine-induced liver injury. (a) Liver confocal intravital microscopy showing that while CBD-treated mice have a well perfused liver vasculature (evidenced by PE-anti-CD31 antibody staining, in red) and absence of necrosis (in green), cocaine-treated mice (75 mg/Kg) had several dark areas in the red channel, which are suggestive of malperfusion caused by necrosis (elicited by DNA staining using Sytox Green). (b) Reduced serum liver transaminase levels and (c) clearance of cardiogreen confirmed inhibition of cocaine-induced liver injury by CBD. However, inhibition of anandamide hydrolysis by URB had no detectable effect. Statistical significance in comparison to controls () and in comparison to vehicle-treated group. A 10x objective was used during imaging. One-way ANOVA with Tukey’s post hoc test. Data are expressed as ( mice/treatment).

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