Expression of Chemokine Receptors on Peripheral Blood T Cells in Children with Chronic Kidney Disease

Table 2

The panel of monoclonal antibodies for lymphocyte subpopulations flow cytometric examination.

Number	FITC	PE	PerCP	PE-Cy7	APC	APC-Cy7
1	CD4; BD	—	CD3; BD	CCR4; BD Pharmingen	—	CD8; BD
2	CD45RO; Dako	CD197 = CCR7; BD Pharmingen	CD3; BD	CD19; BD	CD28; BD Pharmingen	CD8; BD
3	CD4; BD	CD183 = CXCR3; BD Pharmingen	CD3; BD	CD195 = CCR5; BD Pharmingen	CD184 = CXCR4; BD Pharmingen	CD8; BD

BD: Becton Dickinson, San Jose, CA, USA.
BD Pharmingen: Becton Dickinson Pharmingen-Biosciences, La Jolla, CA, USA.
Dako: Dakopatts, Glostrup, Denmark.
FITC: fluorescein isothiocyanate; PE: phycoerythrin; PerCP: peridinin-chlorophyll; PE-Cy7: phycoerythrin-cyanin 7; APC: allophycocyanin; APC-Cy7 allophycocyanin-cyanin 7.